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cd138 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd138 antibody, anti-human, reafinity
    Cd138 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd138/CD138+Antibody%2C+anti-human%2C+REAfinity/custom%40130-115-478%4042502392
    Average 93 stars, based on 10 article reviews
    cd138 antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Systemic deficits in lipid homeostasis promote aging-associated impairments in B cell progenitor development
    Article Snippet: .. Cells underwent viability stain and cell-surface staining against CD19 (viobright 515, (Miltenyi, # 130–112-040)), CD138 (PE, (Miltenyi, # 130–120–810)), as described in Plate A. .. Following that, cells were fixed with True-NuclearTM 1X Fix Concentrate (BioLegend, cat# 73158) for 60 min at room temperature in the dark.

    Article Title: Systemic deficits in lipid homeostasis promote aging-associated impairments in B cell progenitor development.
    Article Snippet: .. Plasma cell panel with fixation: Cells underwent viability stain and cell-surface staining against CD19 (viobright 515, (Miltenyi, # 130–112-040)), CD138 (PE, (Miltenyi, # 130–120–810)), as described in Plate A. .. Following that, cells were fixed with True-NuclearTM 1X Fix Concentrate (BioLegend, cat# 73158) for 60 min at room temperature in the dark.

    Isolation:

    Article Title: Deciphering response dynamics and treatment resistance from circulating tumor DNA after CAR T-cells in multiple myeloma
    Article Snippet: Bone marrow mononuclear cells (BMMCs) were isolated using Ficoll-Paque (Cytiva, Marlborough, MA) according to the manufacturer’s protocol. .. CD138-positive cells were isolated using MACS Cell Separation using antiCD138 microbeads (Miltenyi, Gaithersburg, MD). .. 20 uL of anti-CD138 microbeads were used per total of 2x107 cells with 1:5 dilution by liquid volume.

    Article Title: Targeting the HuR/E2F7 axis synergizes with bortezomib against multiple myeloma.
    Article Snippet: Patient samples Density gradient centrifugation with Ficoll-Paque Plus (Sigma‒ Aldrich, St. Louis, MO) was used to isolate BMMCs from MM patients and PBMCs from healthy donors. .. CD138-positive plasma cells from BMMCs were subsequently isolated with anti-CD138 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. ..

    Article Title: PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism.
    Article Snippet: .. To separate the CD138+ cells (= MM cells) from the CD138- cells, cells were labelled with human CD138 Microbeads (order no. 130–051-301, Miltenyi Biotec, Gladbach, Germany) and isolated by magnetically activated cell sorting according to the manufacturer’s instructions. ..

    Article Title: Targeting the HuR/E2F7 axis synergizes with bortezomib against multiple myeloma
    Article Snippet: Density gradient centrifugation with Ficoll-Paque Plus (Sigma‒Aldrich, St. Louis, MO) was used to isolate BMMCs from MM patients and PBMCs from healthy donors. .. CD138-positive plasma cells from BMMCs were subsequently isolated with anti-CD138 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. ..

    Magnetic Cell Separation:

    Article Title: Deciphering response dynamics and treatment resistance from circulating tumor DNA after CAR T-cells in multiple myeloma
    Article Snippet: Bone marrow mononuclear cells (BMMCs) were isolated using Ficoll-Paque (Cytiva, Marlborough, MA) according to the manufacturer’s protocol. .. CD138-positive cells were isolated using MACS Cell Separation using antiCD138 microbeads (Miltenyi, Gaithersburg, MD). .. 20 uL of anti-CD138 microbeads were used per total of 2x107 cells with 1:5 dilution by liquid volume.

    Clinical Proteomics:

    Article Title: Targeting the HuR/E2F7 axis synergizes with bortezomib against multiple myeloma.
    Article Snippet: Patient samples Density gradient centrifugation with Ficoll-Paque Plus (Sigma‒ Aldrich, St. Louis, MO) was used to isolate BMMCs from MM patients and PBMCs from healthy donors. .. CD138-positive plasma cells from BMMCs were subsequently isolated with anti-CD138 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. ..

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19 + B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19 + B220 + cells were seeded at a density of 2×10 6 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×10 6 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138 + plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: Targeting the HuR/E2F7 axis synergizes with bortezomib against multiple myeloma
    Article Snippet: Density gradient centrifugation with Ficoll-Paque Plus (Sigma‒Aldrich, St. Louis, MO) was used to isolate BMMCs from MM patients and PBMCs from healthy donors. .. CD138-positive plasma cells from BMMCs were subsequently isolated with anti-CD138 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. ..

    Article Title: Systemic deficits in lipid homeostasis promote aging-associated impairments in B cell progenitor development.
    Article Snippet: .. Plasma cell panel with fixation: Cells underwent viability stain and cell-surface staining against CD19 (viobright 515, (Miltenyi, # 130–112-040)), CD138 (PE, (Miltenyi, # 130–120–810)), as described in Plate A. .. Following that, cells were fixed with True-NuclearTM 1X Fix Concentrate (BioLegend, cat# 73158) for 60 min at room temperature in the dark.

    Concentration Assay:

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19 + B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19 + B220 + cells were seeded at a density of 2×10 6 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×10 6 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138 + plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    FACS:

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19 + B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19 + B220 + cells were seeded at a density of 2×10 6 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×10 6 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138 + plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism.
    Article Snippet: .. To separate the CD138+ cells (= MM cells) from the CD138- cells, cells were labelled with human CD138 Microbeads (order no. 130–051-301, Miltenyi Biotec, Gladbach, Germany) and isolated by magnetically activated cell sorting according to the manufacturer’s instructions. ..

    Cell Isolation:

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19 + B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19 + B220 + cells were seeded at a density of 2×10 6 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×10 6 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138 + plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..



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